Recombinant Protein L Protein, His Tag

Source : 

Recombinant Protein L, His Tag, also known as Recombinant Peptostreptococcus magnus Protein L, was expressed in E.Coli at ACRObiosystems.

Molecule : Protein L

Synonyms : RPL,Protein L

Format : Powder

Category : MABSol® Affinity Media Ligands

Accession : N/A

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : N/A

Characteristics :

Recombinant Protein L, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 45 kDa polypeptide. Protein L Protein can bind to VL-Kappa.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : 100 mM NaH2PO4, pH8.5

Description :

Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

References :

(1) L Bjorck, 1988, The Journal of Immunology, Vol 140, Issue 4: 1194–119
(2) Nilson, B.H., et. al., 1993, J. Immunol. Methods164, 33-40.