SUMO Activating Enzyme (E1)

PDF

E-315

25 μg

Brand

Boston Biochem

Description

SUMO Activating Enzyme (E1)

Catalog #: E-315

SUMO Activating Enzyme (E1)
Product Source: 

human recombinant

Also known as: SUMO Activating Enzyme (SAE1/SAE2), or UBA2

Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (SAE1/SAE2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high- energy thiolester bond with the cysteine residue of SAE2 and the concomitant release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9.

Product Information
Quantity: 

25 μg

Stock: 

X mg/ml (X μM) in 50 mM Hepes pH 8, 200 mM NaCl, 1 mM DTT, 10% glycerol. Actual concentration will vary with specific lot number.

MW: 

110 kDa

Purity: 

> 90% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Application

Reactivity

Photos

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