SUMO Activating Enzyme (E1)

PDF

E-311

25 μg

Brand

Boston Biochem

Description

SUMO Activating Enzyme (E1)

Catalog #: E-311

SUMO Activating Enzyme (E1)
Product Source: 

S. cerevisiae

Also known as: SUMO Activating Enzyme (Aos1/Uba2)

Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (Aos1/Uba2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1 (also SUMO-2 and SUMO- 3), forming a high-energy thiolester bond with the cysteine residue of Uba2 and the release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO-1 is transferred to Cys93 of UbcH9.

Product Information
Quantity: 

25 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 

114 kDa

Purity: 

> 90% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vi

Application

Reactivity

Photos

BACK TO LIST
回到產品列表