UbcH7, Dominant Negative

PDF

E2-642

100 μg

Brand

Boston Biochem

Description

UbcH7, Dominant Negative

Catalog #: E2-642

UbcH7, Dominant Negative
Product Source: 

human recombinant

UbcH7 mediates the selective degradation of short-lived and abnormal proteins and is highly homologous to UbcH5. UbcH7 interacts with the HECT domain of E6-AP and the RING domain of c-Cbl, and can mediate the multi-ubiquitination of many different types of protein substrates. This enzyme has a mutation of the active site from cysteine to serine which abolishes the ability of UbcH7 to transfer ubiquitin to an accepting E3 protein. Ideal for use as a negative or competitive control, or to study protein-protein interactions.

Product Information
Quantity: 

100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.

MW: 

18 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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