YeastHIGHTM Advanced Protein Expression Package 增強蛋白表現包裝

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SC1346

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Description

GenScript專有YeastHIGHTM技術結合的優點，細菌和真核生物表達系統。我們的酵母表達系統採用穩定和持久的生產菌株，類似於哺乳動物蛋白加工，結合了多種功能的高產量，高生產力，並大幅削減成本和時間。

GenScript開發了一個平台，產生的抗體在酵母表達系統。相似性酵母和哺乳動物系統重組抗體的表達，降低成本與製造抗體的重組真核表達系統。

GenScript的工程增強蛋白表達技術，使高成功率超過90％的多種多樣的重組蛋白，我們的專有YeastHIGHTM技術殖。使用酵母表達系統或酵母分泌信號可以生產成功很多細菌分泌的蛋白質表達系統產生的不溶性或無效包涵體。

Milestones Specifications Deliverables Timeline *
1. Subcloning
  • Subcloning into two appropriate expression vectors
  • Plasmid maxi preparation
  • Linearize the constructs
Construct(s) report
2 weeks
2. Expression optimization and evaluation
  • Transformation the linear construct into the appropriate host strain
  • Confirmation of positive transformants via PCR
  • Expression optimization and confirmation
    i: Screening of transformants with multiple gene-insert with in-house YeastFast technology
    ii: Media formulation and inducer concentrations
    iii: SDS-PAGE gel and/or Western blot
Expression data
3–5 weeks
3. Protein expression and purification
  • Small-scale expression from one liter
  • One-step purification, or additional purification to reach purity of 90% or higher
Purified soluble protein
QC data
2 weeks
4. Large-scale protein production and purification (Optional)
  • Large-scale production up to 500 L (Employs fermentors and TFF large-scale filtration system)
  • Deliverables available at >90% purity or higher
  • Removal of tags
Yeast paste/
Purified proteins
QC data
2–3 wee

上述是典型的周轉時間。請注意，額外要求可能帶來額外的步驟，同樣的需要更長時間處理。

Application

Reactivity

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