Abbiotec | 適用於各種應用的Human IgE和二抗

Abbiotec | 適用於各種應用的Human IgE和二抗

2022.08.24

用於肥大細胞(mast cell) 去顆粒作用試驗的Human IgE

 

Cat. No. 250206 Human IgE (biotinylated)成功用於使human mast cell敏感化,並在將streptavidin添加到細胞培養基中時觸發去顆粒反應。

 

Human IgE 產品已用於刺激下列細胞:

  • human mast cells
  • human airway smooth muscle cells (HASM)
  • human bronchial/tracheal smooth muscle cells (B/TSM)
  • acute myelogenous leukemia blasts (AML)
  • human neutrophils

 

Biotinylated human IgE and streptavidin trigger degranulation  of  LAD2 human mast cells. LAD2 cells were sensitized overnight with 0.1 ug/ml biotinylated human IgE (Abbiotec Cat. No. 250206). Cells were incubated with streptavidin (SA) for 30 min at 37°C. Degranulation was monitored by beta-hexosaminidase release into the supernatant calculated as a percentage of total content.
Data kindly provided by Prof. Fernando de Mora, Universidad Autonoma de Barcelona, Spain.

Human IgE

 

Cat. No. 250202是一種非免疫humam IgE,是由健康的人類分離出來的單株雜交瘤在體外生產。IgE 是由親和法純化的完全人源抗體,具有ε heavy chain和κ light chain。IgE 的單株來源確保抗體特性的一致性以及避免和其他種類的Ig污染。Human IgE 產品可作為定量 IgE 試驗和細胞敏感性試驗中的標準品。Abbiotec能提供已純化、azide free和biotinylated形式的human IgE。

更多資訊請洽亞旭生物科技,可獲得全產品的資訊以及諮詢,更多關於human IgE以及二抗產品列表可以下載產品手冊

Quality control of Human IgE preparation  from hybridoma (lane 2) vs. IgE sample purified from myeloma patient serum (lane 3). Molecular weight markers are shown in lane 1.


 

Control和二級抗體

 

瀏覽Abbiotec提供的 ELISA、Western blot、IHC和Flow應用開發的人、小鼠、兔和大鼠物種的目錄抗體。下面列出Isotype controls、human IgE 和二級抗體。

Cat. No.

Product Name

250202

Human IgE, 0.1 mg

250204

Human IgE, 1 mg

250203

Human IgE, azide free, 0.1 mg

250205

Human IgE, azide free, 1 mg

250206

Human IgE, biotin-conj. , 0.1 mg

252240

Anti-goat IgG Antibody (HRP), 0.5 mg

250114

Anti-human IgA Antibody, 0.1 mg

250140

Anti-human IgE Antibody, 0.1 mg

250116

Anti-human IgG Antibody, 0.1 mg

250928

Anti-human IgM Antibody, 0.1 mg

250929

Anti-human IgM Antibody (PE), 2 ml

250930

Anti-human IgM Antibody (FITC), 2 ml

254517

Anti-human IgM Antibody (HRP), 0.1 mg

250987

Anti-human kappa Ig Antibody, 0.1 mg

251043

Anti-human kappa Ig Antibody (FITC)2 ml

251099

Anti-human kappa Ig Antibody (PE), 2 ml

252241

Anti-human lambda Ig Antibody, 0.1 mg

252242

Anti-human lambda Ig Antibody (FITC)

252243

Anti-human lambda Ig Antibody (PE) 2 ml

250137

Anti-mouse IgG Antibody (HRP), 1 mg

250138

Anti-mouse IgG Antibody (FITC), 1 mg

250139

Anti-mouse IgG Antibody (Fab’)2 (FITC)

252237

Anti-rabbit IgG Antibody (HRP), 1 mg

252238

Anti-rabbit IgG Antibody (FITC), 1 mg

250842

Anti-rat IgA Antibody, 0.1 mg

250115

Anti-rat IgG Antibody (FITC), 0.1 mg

252239

Anti-rat IgG Antibody (HRP), 1 mg

250446

Anti-rat kappa Ig Antibody, 0.2 mg

250198

Control Mouse IgG1, 0.1 mg

250207

Control Mouse IgG1 (PE), 2 ml

250199

Control Mouse IgG2a, 0.1 mg

252507

Control Mouse IgG2a (FITC), 2 ml

252508

Control Mouse IgG2a (PE), 2 ml

252509

Control Mouse IgG2b, 0.1 mg

252510

Control Mouse IgG2b (FITC), 2 ml

252511

Control Mouse IgG2b (PE), 2 ml

254513

Control Rabbit IgG, 10 mg

新推出:  Rabbit, Goat. LLama正常血清和血漿作為blocking agent和controls

 

Cat. No. 250202 Human IgE for new assay development
Chang C. et al. 2014. Analyst. PMID 24821053. Label-free colorimetric aptasensor for IgE using DNA pseudoknot probe.

 

Cat. No. 250203 Human IgE Azide Free for mast cell sensitization
Mills K. et al. 2014. Allergy. PMID 24697338. Vitamin E, gamma-tocopherol, diminishes ex vivo basophil response to dust mite allergen.

 

Cat. No. 250205 Human IgE Azide Free (1 mg) for IgE receptor binding studies
Sharquie I. et al. 2013. BMC Immunol. PMID 24330349. An investigation into IgE-facilitated allergen recognition and presentation by human dendritic cells.

 

Cat. No. 250206 Human IgE (biotinylated) for mast cell degranulation studies
Ainsua-Enrich E. et al. 2012. J Immunol. PMID 22896635. The Adapter 3BP2 Is Required for Early and Late Events in FcεRI signaling in Human Mast Cells.

Janson C. et al. 2012. PLoS ONE. PMID 23185384. RIN3 Is a Negative Regulator of Mast Cell Responses to SCF.

Serra-Pages M. et al. 2012. J Leukoc Biol. PMID 22859831. E-prostanoid 2 Receptors Dampen Mast Cell Degranulation via cAMP/PKA-Mediated Suppression of IgE-Sependent Signaling.

 

Cat. No. 250928 Anti-Human IgM Antibody
Dong ZX. et al. 2013. World J Gastroenterol. PMID 23901222. Resistin mediates the hepatic stellate cell phenotype.

 

Cat. No. 252237 Anti-Rabbit IgG HRP-Conjugated
Meng Q. et al. 2014. Tumour Biol. PMID 25168370. HMGB1 promotes cellular proliferation and invasion, suppresses cellular apoptosis in osteosarcoma.

 

Cat. No. 252240 Anti-Goat IgG HRP-Conjugated
Tan J. et al. 2011. Tumour Biol. PMID 21874375. Expression and Significance of Six1 and Ezrin in Cervical Cancer Tissue.

 

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