外泌體(Exosome)是由細胞分泌奈米等級脂雙層的囊泡，為細胞外囊泡 (Extracellular vesicles, EVs) 的一種。與其他的囊泡區分，其直徑大小介於約40-120nm。在組成物中，研究發現外泌體包含核酸、蛋白、脂質、細胞激素、轉錄因子受體等生物活性分子。細胞可以藉由外泌體進行細胞間的溝通或是生物分子的傳遞，在生理、病理的機制中可以說是非常重要。隨著對外泌體更深入的研究，可將外泌體相關蛋白作為診斷疾病的生物標記物、藥物傳遞載體、癌症疫苗等應用。
Abcam作為全球最優質的實驗試劑領導品牌，可以提供多樣的Exosome實驗研究工具，包含Exosome分離與檢測的Isolation Kit / Beads、定量Exosome的ELISA Kit、檢測Exosome蛋白的Antibody Panel、可在多種實驗應用作為control的Exosome Standard。
Exosome Panel
u 一次囊括exosome熱門的markers，內含有Calnexin、CD9、CD63、CD81、Hsp70和TSG101各20ul的重組抗體，多種markers一次滿足。
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Cat. No. |
Product |
Components |
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Exosome Panel |
Anti-Calnexin antibody (ab133615) |
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Anti-CD9 antibody (ab263019) |
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Anti-CD63 antibody (ab134045) |
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Anti-CD81 antibody (ab109201) |
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Anti-Hsp70 antibody (ab181606) |
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Anti-TSG101 antibody (ab125011) |
Isolation and Analysis Kit
u 針對exosome markers設計含有capture antibody以及帶有PE的detection antibody，可用於分離exosome並flow cytometry分析。
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Cat. No. |
Product |
Sample |
Components |
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Exosome Isolation and Analysis Kit |
Cell culture |
CD63+ Capture Beads / Anti-CD9 PE |
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Plasma |
CD9+ Capture Beads / Anti-CD81 PE |
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Urine |
CD63+ Capture Beads / Anti-CD9 PE |
Capture Beads
u 多種exosome marker capture beads，可用於分離/檢測exosome
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Cat. No. |
Product |
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CD63 Exosome Capture Beads |
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CD81 Exosome Capture Beads |
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CD9 Exosome Capture Beads |
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CD326 Exosome Capture Beads |
ELISA Kit
u 內含Lyophilized Exosome Standards的雙夾心ELISA，可執行exosome的定量分析。
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Cat. No. |
Product |
Sample |
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Overall
Exosome Capture and Quantification ELISA Assay Kit |
Cell culture supernatant |
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Serum |
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Plasma |
Standard
u 可用作為多種實驗應用(FACS、WB、ELISA等定量exosome實驗)的positive
controls以及校正標準品。多種生物來源(cell culture supernatant、human plasma、serum、urine和saliva)的exosome可供選擇。
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Cat. No. |
Product |
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Lyophilized Exosome Standard (Human
Plasma) |
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Lyophilized Exosome Standard (100 µg,
Human Serum) |
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Lyophilized Exosome Standard (U87 MG) |
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Lyophilized MCF7 Exosome Standards |
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Lyophilized PC-3 Exosome Standards |
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B16F10 Fluorescent Exosome Standard |
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Plasma Fluorescent Exosome Standard |
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Lyophilized Exosome Standard (100 µg,
Human Urine) |
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Lyophilized HT-29 Exosome Standards |
