已通過基於細胞的分析驗證的高生物活性Siglec-15蛋白
High bioactivity Siglec-15 protein verified by cell-based assay
ACROBiosystems developed high bioactivity Siglec-15 to help drug development.
Features
- Validated by Cell-based assay, BLI, SPR and ELISA
- Protocols are offered for free
- More species and tags to choose
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Product List
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Assay Data
❇Cell based assay
Fig.1 Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) inhibits Anti-CD3-induced proliferation of PBMC. The ED50 for this effect is 0.48-0.57 μg/mL.
>>> Protocol
❇BLI
Fig.2 Loaded Anti-Siglec-15 MAb (Mouse IgG) on AMC Biosensor, can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 59.7 pM as determined in BLI assay (ForteBio Octet Red96e).
>>> Protocol
❇SPR
Fig.3 Immobilized Neu5Ac(a2-6)GalNAc-PAA-biotin on SA Chip can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 4.49 μM as determined in a SPR assay (Biacore T200).
>>> Protocol
❇ ELISA
Fig.4 Immobilized Mouse Human chimeric MAb (5G12, Human IgG1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) with a linear range of 0.4-3 ng/mL.
>>> Protocol
* If you would like ACRO to add more experimental design and application data of Siglec-15 protein, please contact us.
* For more Siglec proteins, please click the molecule name below to find more.
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Customer Reviews
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