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Finding a super stable trimeric S protein?
The S protein exists as a homotrimer on the surface of coronavirus. Trimeric S protein with correct conformation and high stability is of great significance for antiviral drug screening, vaccine development and serological test kit development.
ACROBiosystems has developed super stable trimeric S proteins with high purity, correct molecular weight and natural polymeric form rigorously verified by SDS-PAGE, SEC-MALS and NS-EM, respectively.
Product List
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Cat.No.
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Species
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Host
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Product description
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SPN-C82E9
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SARS-CoV-2
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HEK293
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Biotinylated SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag, Super stable trimer (MALS verified)
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SPN-C52H9
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SARS-CoV-2
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HEK293
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SARS-CoV-2 (COVID-19) S protein (R683A, R685A), His Tag, Super stable trimer (MALS & NS-EM verified)
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Assay data
※ The purity, molecular weight and polymeric form were rigorously verified by SDS-PAGE, SEC-MALS and NS-EM.
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Fig 1. The purity of SARS-CoV-2 S protein (R683A, R685A), His Tag, Super stable trimer (Cat. No. SPN-C52H9) was more than 90% verified by SDS-PAGE under reducing (R) condition. The molecular weight was around 550-660 kDa confirmed by SEC-MALS. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature verified by negative stain electron micrography.
>>> Protocol
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※Bioactivities of binding to ACE2 protein and anti-SARS-CoV-2 neutralizing antibody were verified by ELISA
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Fig 3. Immobilized Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.5-16 ng/mL.
>>> Protocol
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