Finding purer CD3 proteins in 1:1 heterodimer for your BsAb development? Get the free sample now!
CD3-targeted therapeutic antibody usually recognizes the CD3ε in the heterodimer complex to activate the anti-tumor activity of T cells. However, in the process of recombinant expression, CD3E and CD3D can randomly form incorrect heterogeneous complexes, leading to the loss of bioactivity and high batch-to-batch inconsistency.
ACROBiosystems has developed a series of confirmed homogeneous CD3δ/CD3ε and CD3γ/CD3ε proteins with good bioactivity.
· Purer 1:1 heterodimers verified by non-reducing electrophoresis and MALS
· Show good performance in different applications in therapeutic antibody development
· Used for the development of a bispecific antibody in clinical phase successfully
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Product List
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Assay Data
>>> Purer 1:1 heterodimer verified by non-reducing electrophoresis and MALS
Fig.1 Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) on SDS-PAGE under reducing (R) and non-reducing（NR）condition. The purity of the protein was more than 85% and around 35-43 kDa verified by SEC-MALS.
>>> High bioactivity and batch-to-batch consistency
Fig.2 Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.2-6 ng/mL, and batch differences EC50<0.0001 μg/mL.
>>> Applied for antibody drug development, screening, identification and quality control
Fig.3 Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) with an affinity constant of 31.8 nM as determined in a SPR assay (Biacore T200).
>>> Applied for clinical PK assay
Fig.4 Immobilized Human BCMA, His Tag (Cat. No. BCA-H522y) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) in 10% human serum and then add Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 15 ng/mL.
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