ACROBiosystems | 【新品上市】高效助益 CAR 檢測的兔抗 218 Linker 抗體

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近年來，CAR-T（嵌合抗原受體T細胞）療法因其在治療復發難治性血液腫瘤方面展現出顯著療效，成為最受關注的免疫細胞治療技術之一。CAR結構中，柔性Linker作為scFv中的可變重鏈（VH）與可變輕鏈（VL）之間的柔性橋樑，起著至關重要的作用。目前最常用的柔性Linker包括G4S Linker與Whitlow/218 Linker。其中，218 Linker由Whitlow等人於1993年首次報導，其氨基酸序列為GSTSGSGKPGSGEGSTKG。該Linker因其良好的柔性和穩定性，被廣泛應用於各類CAR構建中。

在CAR-T療法逐步臨床應用過程中，準確的CAR表達檢測至關重要。通過檢測CAR表達水準，可以量化CAR-T細胞中的CAR陽性率，進而評估其抗腫瘤能力。為實現高效且標準化的CAR檢測，我們推出了一系列兔抗CAR Linker抗體，該抗體專門識別CAR結構中的Linker區域，提供了更高的檢測通用性和一致性的CAR檢測工具，顯著提升了CAR-T產品的品質控制和功能驗證。

產品優勢

• 多靶點通用——專一結合VL-VH間的Whitlow/218 linker，不區分靶點
• 多CAR結構通用——專一結合VL-VH間的Whitlow/218 linker，適用於絕大部分CAR結構
• 種屬、亞型通用——不受抗體亞型和種屬限制
• 體內外通用——不受抗原封閉和血清影響
• 高親和力——自研單B兔抗開發平臺
• 高特異性——PBMC非特異結合低於0.5%

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Verification Data

• Validated by flow cytometry, our Anti-CAR Linker Antibodies demonstrate high specificity in binding to CAR-like cells.

Flow cytometric analysis of Anti-CD19 CAR-293/Anti-MSLN CAR-293/Anti-CD2 CAR-293 cells staining with PE-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-PFMY25) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity.

• Extremely low non-specific signal: validated by PBMC double staining

Non-specificity of PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with APC-Labeled Monoclonal Anti-Human CD3 Antibody and PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-PCFMY3) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both APC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).

• Superior positive Signal: outperforming competitors

Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with PE-Labeled Monoclonal Anti-Whitlow 218 Linker Antibody, Rabbit IgG (P3C09) (Cat. No. 218-PCFMY3) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and Competitor C. PE signal was used to evaluate the binding activity. The biological activity level of 218-PCFMY3 is superior to Competitor C (Routinely tested).

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