IgGs experience high serum half-life due to the protective FcRn recycling pathway. Adapted from Roopenian et al., 2007.
為評估抗體藥物的半衰期，我們提供了一系列與FcRn相關的產品和服務，包括：
FcRn蛋白：二聚體結構及高純度經SEC-MALS驗證；高活性經SPR/BLI驗證。
FcRn結合試劑盒（TR-FRET）：無需洗滌步驟，可進行高通量篩選；1完成實驗。
FcRn穩定細胞株：穩定傳代超過20代；表達活性經FACS驗證。
Verification Data
FcRn 二聚體結構經SEC-MALS驗證
The purity of Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-C82W9) is more than 95% and the molecular weight of this protein is around 43-53 kDa verified by SEC-MALS.
FcRn與Herceptin親和力經SPR驗證
Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No.FCM-H82W7) on SA Chip can bind Herceptin® with an affinity constant of 0.267 μM
The half-lives of these 3 monoclonal antibodies currently in clinical use generally correlate with the binding affinity to FcRn. The kit has been used to detect 3 FDA approved antibody drugs of different binding affinity to FcRn, and the IC50 trends are consistent with affinity constant from SPR as well as the actual in vivo half-life published.
