Conjugating a fluorophore like ATTO directly to the primarily means you don’t have to worry about a secondary antibody (or the extra washing steps that go along with it). Not only is this convenient and quicker, it means you don’t need to worry about the secondary binding non-specifically.
Guinea pig-raised antibodies
As you’re well aware, as the number of targets in your multiplex goes up, so does the complexity of antibody selection. Our guinea-pig raised antibodies, make that easier by giving your more options.