Anatrace |新一代nanodisc-forming SMA聚合物:膜蛋白研究的最新工具

• Four, novel, modified SMA polymers, designed to form planar lipid bilayer nanodiscs [1]
• Direct, detergent-free systems for the extraction of membrane proteins (MPs) in their natural membrane environment
• Used for the reconstitution of MPs into synthetic lipid nanodiscs
• Demonstrated to solubilize well-folded MPs [1]
• Good for structural and functional studies of MPs

Why use them?

• Increased stability in presence of divalent cations: up to 200 mM Ca²⁺ or Mg²⁺ (see Fact Sheets or [2])
• These new molecules cover the full range of pH stabilities [2]
• Easy nanodisc size control: adjust by altering the polymer:lipid ratio
• Capable of reconstituting MPs with large, charged, soluble domains [1]
• Easy nanodisc formation protocol: add directly to membranes or lipids, incubate, then proceed to purification or other techniques
• Compatible with most protein purification and characterization techniques

SMA Application Focus:

• Studies of membrane proteins in native lipid environments
• Structural studies of large membrane proteins and complexes
• Studies of protein:lipid interactions
• Work with proteins that are unstable in detergents
• Solubilization of membrane proteins with large charged domains