不明原因的兒童重症急性肝炎-高純度致病性核酸的分離
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幾個月以來，多個國家發現幾起病因不明的兒童急性肝炎疑似病例和死亡病例，其中認為是腺病毒和 SARS-CoV-2 的機率非常高。而mNGS技術可辨識多種病原體，如細菌、真菌、病毒和寄生蟲，也為肝臟感染的精準診斷提供非常大的幫助。因此，用於建庫斷裂 DNA/cDNA 序列的高回收率和純度的分離方式是非常重要的，目前磁珠對於破碎DNA有著更高純度回收的理想工具，適用於 mNGS 的肝炎診斷。 |
| CD Bioparticles 能提供 MagIso™ DNA & RNA Magnetic Silica Particles，適用於從患者血液、呼吸道分泌物、糞便、體液、肝組織和其他樣品中分離核酸。這些顆粒根據其不同的silica塗層，可以在高鹽條件下與核酸緊密結合。最多可從 200 µL 全血中萃取 10 µg genomic DNA，並回收 85% 以上的genomic DNA。同時silica particles還適用於自動化96孔核酸萃取儀器，可以進行高通量篩選。因此CD Bioparticles的silica particles在核酸萃取方面具有強大的應用價值，是 PCR、mNGS 和其他分子生物學實驗樣品製備的理想選擇。 |
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References:
1. Li H, et al. Detection of pulmonary infectious pathogens from lung biopsy tissues by metagenomic next generation sequencing. Front Cell Infect Microbiol, 2018, 8: 205.
2. Liu H, et al. Application of mNGS in the etiological analysis of lower respiratory tract infections and the prediction of drug resistance. Microbiol Spectr, 2022, 10(1):e0250221.
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優勢:
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操作簡單：只需裂解、結合、洗滌和洗脫步驟 |
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靈敏度高：適用於法醫學樣本及其他微量DNA萃取 |
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高純度：分離的核酸可立即用於下游應用
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穩定懸浮：無磁力時沉降率低
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自動化、高通量：可配合96孔核酸自動萃取儀器
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高重複性：CV<5%
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