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| Overview |
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| Creative Biogene is dedicated to delivering high-quality, rapid, and cost-effective stable cell line generation services to support protein expression, gene knockdown, and genome editing for specialized research needs. With a wealth of experience, our cell culture scientists focus on fine-tuning every parameter to meet specific requirements, using proprietary expression vectors, advanced high-throughput screening technologies, and animal-component-free processes to generate reliable and adaptable cell lines in reduced timelines. |
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| Service Highlights |
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Comprehensive Solutions for Stable Cell Line Generation |
| Creative Biogene offers a robust range of services for stable cell line generation, covering applications such as protein expression, gene knockdown, genome editing, and circRNA overexpression. Our extensive experience spans over 150+ cell lines, including H1299, MCF-7, HEK293, 3D4/2, HT-29, and more, ensuring reliable and scalable solutions for diverse cell lines. |
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| Platform Expertise and Process Optimization |
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| Our service approach is based on a three-phase model: |
| 1. Vector Design and Optimization |
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Codon optimization for enhanced expression. |
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Promoter selection tailored to specific expression needs. |
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Stability elements and advanced selection markers integration. |
| 2. Cell Line Development and Screening |
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Strategic host cell line selection. |
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Transfection/transduction optimization. |
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High-throughput clone screening and expansion. |
| 3. Advanced Gene Editing |
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CRISPR/Cas9 gene editing for precise knockout/knock-in. |
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Custom RNA interference for targeted gene knockdown. |
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| Core Services |
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| Gene Expression Cell Lines |
| Flexible cassette options, comprehensive clone selection, and high-level protein production for research applications. |
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| Gene Knockdown Cell Lines |
| Precise shRNA design and validation for sustained gene silencing and extensive screening potential. |
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| CircRNA Overexpression |
| Specialized service for disease research using structurally stable circular RNAs—comprehensive design, construction, and validation. |
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| Quality Control |
| We maintain stringent quality control throughout the development process: |
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Genetic Validation: Sequence verification, copy number analysis, and genomic integration confirmation. |
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Expression Validation: Quantification of RNA and protein expression, stability assessments, and functional verification. |
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Microbial Testing: All cell lines are mycoplasma-free, and sterility tests are conducted as part of our rigorous validation process. |
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| Technical Process |
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| Our services are designed to ensure smooth, efficient, and reliable results. We support every step of the cell line development process: |
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Precise vector and gene target selection |
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Custom strategy development |
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Construct generation for expression/knockdown |
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High-throughput vector production |
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Stable clone establishment |
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Comprehensive molecular validation (Western blot, PCR, sequencing) |
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Continuous technical guidance |
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Detailed protocol and data documentation |
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 Schedule an Approintment |
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