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Green fluorescent protein (GFP) has become a commonly used molecular imaging tool in biology, genetics, medicine, and chemistry. The GFP gene can be efficiently integrated into expression vectors without substantially increasing the vector’s size. This feature allows the vector construction to combine a gene of interest, for instance, a therapeutic gene, and the GFP gene to be a visual marker, without losing infection or transfection efficiencies. In many cases, the use of GFPs as a marker for efficient biological integration provides an advantage over the more traditional antibiotic selection. Successfully transfected cells expressing GFP can be quickly sorted by flow cytometry for immediate therapeutic use.
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Based on years of experience and in-depth investigation, Creative Biogene has established a large number of stable GFP reporter cell lines. The GFP gene is stably integrated into the target cell genome to ensure stable reporter gene expression during continued passaging in culture. Importantly, an antibiotic selection gene (either puromycin or neomycin) is coupled to the reporter gene of interest to ensure that the cells express high levels of the desired reporter gene. Our stable GFP reporter cell lines are perfectly suited for in vivo imaging studies, where the high reporter gene expression facilitates the detection of fewer implanted cells.
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