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Over recent years, the IL-18 protein has garnered significance in the field of immunological research and therapy. This protein not only participates in various inflammatory responses but also plays a crucial role in anti-tumor immunity; IL-18 enhances the activity of CD8+ T cells and NK cells, promotes the secretion of IFN-γ, and offers a promising avenue for cancer immunotherapy.
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Why Choose Our GMP-grade IL-18 Protein?
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High-Quality Production
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Robust Source: Produced using HEK293 cells to ensure the natural activity and structure of proteins.
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High Purity: Features purity levels >95%, verified by SDS-PAGE, meeting stringent quality standards.
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Low Endotoxin: Features an endotoxin level <100 EU/mg, ensuring safety in experiments and applications.
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Featured Product
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Cat.No.
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IL18-153HG
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Product Name
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GMP Recombinant Human IL18 Protein
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Source
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E. coli
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Activity
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Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is ≤ 2 ng/ml.
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Endotoxin:
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35,380 units/mg
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Purity
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<1.0 EU per 1 μg of the protein by the LAL method.
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SDS-PAGE
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Activity
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HPLC
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SEC-HPLC
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Defined Biological Activity
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The biological activity of our IL-18 proteins reaches 1.0×106 IU and is validated through KG-1 cell-induced IFN-γ secretion experiments.
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Diverse Applications
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Inflammation Research: Used to study the mechanisms of IL-18 in inflammatory diseases such as inflammatory bowel disease and osteoarthritis.
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Cancer Therapy: Used for exploring the potential of IL-18 in regulating tumor immune microenvironments and immunotherapy.
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Customer Feedback
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Performs well in inflammatory models, with high stability in serum; suitable for ELISA detection.
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Research Cases
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Case 1: Inflammatory Bowel Disease Study
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In one study, IL-18 was shown to alleviate symptoms of colitis induced by a guar gum diet. The research indicated that IL-18 reduced an inflammatory response by modulating the gut microbiota and enhancing intestinal barrier function.
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Figure 1. IL-18 expression in jejunum of indicated mice by Western blotting. (Xuesen Zheng, et al. 2021)
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Case 2: Osteoarthritis Study
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Researchers found that the expression of IL-18 was significantly elevated in the synovial tissue of patients with osteoarthritis. Further experiments demonstrated that IL-17 promotes the production of IL-18 through the MEK/ERK/miR-4492 signaling pathway, highlighting the important role of IL-18 in the progression of osteoarthritis.
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Figure 2. IL-18 is upregulated in clinical synovial tissue from OA patients. (Lee, T., et al. 2024）
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Case 3: NK Cell Cultivation
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In a large-scale NK cell cultivation study, the combined use of IL-18 and IL-12 significantly enhanced the proliferation and cytotoxicity of NK cells. The research showed that this synergistic effect can effectively boost the anti-tumor activity of NK cells, providing strong support for NK cell-based immunotherapy.
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Figure 3. PBMC and purified NK cells were cultured with medium (Control) or a maximal concentration of IL-12 and IL-18 without or with TL1A (50 ng/ml), and their cytotoxicity was tested against the NK-sensitive cell line K562 in 51Cr-release assays and expressed in lytic units (LU30, left panels). (Stephanie C. Heidemann, et al. 2010)
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Contact us now to get more product information or request a trial sample, and advance your research and application process!
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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-559-9269 / 1-516-512-3133
Fax: 1-631-938-8127 Germany 65830 Kriftel, Gutenbergstraße 5. Frankfurt am Main, Germany
+491793975992
Email: info@creative-biomart.org
Global Locations CREATIVE BIOMART INC.
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