CUSABIO | 如何挑選用於蛋白分析的標記抗體 (Tag Antibodies)

Consists of six consecutive histidines and is commonly used for protein purification by metal ion affinity chromatography (IMAC). Advantages include mild purification conditions, small tags and low impact on protein function.

Glutathione S-transferase, advantages include enhanced solubility of exogenous proteins, maintenance of protein antigenicity and biological activity, but higher molecular weight may affect protein function.

Maltose-binding protein that contributes to increased proteolysis, especially for eukaryotic proteins. Can be purified by starch affinity chromatography, but larger tags may have an effect on protein structure/function.

Maltose-binding protein that contributes to increased proteolysis, especially for eukaryotic proteins. Can be purified by starch affinity chromatography, but larger tags may have an effect on protein structure/function.

High affinity, short sequence, does not interfere with the function of the target protein, can be efficiently purified by affinity chromatography, and can be excised by enterokinase to obtain the natural protein.

Contains 11 amino acids and is commonly used in Western-blot hybridization techniques, immunoprecipitation, etc. It is suitable for detecting the expression of recombinant proteins in cells.

Derived from erythrocyte agglutinin of influenza virus, 9 amino acids long, with little effect on the spatial structure of the target protein, easy to detect by Anti-HA antibody and ELISA.

Green fluorescent protein, widely used in live cell imaging, with different excitation and emission wavelengths, suitable for multicolor labeling and real-time monitoring of protein expression.