CUSABIO | 跨膜蛋白表達大師-CUSABIO體外大腸桿菌表達系統

In vitro E.coli expression system, also known as cell-free protein expression system or the in vitro translation system, simulates in vivo cells and reproduces the intracellular protein transcription and translation process. It needs the existence of various materials required for protein synthesis, including energy, transcription factors, and translation factors, etc.

The system is particularly suitable for the expression of transmembrane proteins and toxic proteins. Its feature includes short cycle and high-throughput. CUSABIO has mastered the full set of core technology of E.coli cell-free expression system, all core components are produced in house, and the reaction system contains more than 40 ingredients, which are easy to be adjusted and optimized.

Service Process

Since the establishment of this platform in 2015, 162 proteins have been successfully produced with yield of mg/ml, which contains 99 transmembrane proteins with 1-12 transmembrane domains and toxic proteins that are difficult to express in traditional E.coli expression systems. We have also produced high molecular weight proteins (130 kDa -140 kDa) that contain multiple transmembrane domains.

Project Showcase

Case 1: The following three items are proteins with 5, 6 and 7 transmembrane domains separately.

Case 2: The project was a 9 transmembrane protein.

Case 3: The protein in this project had a very low yield. Through optimization of different N-terminal peptides, the yield was improved dramatically, as shown in Figure 5.