Eurogentec: 用於 COVID 檢測的優化RT-qPCR試劑

Eurogentec: 用於 COVID 檢測的優化RT-qPCR試劑

2021.09.22

本文轉載至原廠電子報: Eurogentec  https://www.eurogentec.com/en/  

 

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Takyon Ultra

 

 

A unique One-Step RT-qPCR 4x MasterMix
readily available

We have developed Takyon Ultra – for the sensitive and specific detection of viral RNA templates such as that from SARS-CoV-2.

 

 

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Highly efficient on SARS-CoV-2 RNA templates

 

 

Increased specificity

Increased specificity

No amplification signal is observed for a known COVID negative sample with the Takyon Ultra (yellow) while competitor T reagents gives false positive signal. In addition, the virus is detected with a brighter signal for the 2 positive patient samples when using Takyon Ultra . 

Figure 1. Sarbeco E gene (SARS-Cov-2 RNA viral target) detection in samples from known COVID-19 positive (patients 1 & 2) or negative (patient 3) patients.

 

 

 

High sensitivity

Takyon Ultra (yellow) demonstrates a better linear dynamic range than competitor T (dark blue) and better signal than competitor B (light blue) reagents. 

Figure 2. Linear dynamic range in 10-fold dilutions from 500,000 down to 5 copies of SARS-COV-2 RNA Control (TWIST Bioscience #MT007544.1).

High sensitivity

 

 

 

Optimized stability

Optimized stability

Takyon Ultra (yellow) maintains consistent amplification results after 24h at -20°C (dark blue, recommended storage conditions), 4°C (light blue) or 20°C (yellow).

Figure 3. 10-fold linear range from 100 000 down to 10 copies of SARS-COV-2 RNA Control (TWIST Bioscience #MT007544.1) under different storage temperatures for 24h.

 

 

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