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Oncorhynchus keta Lectin (CSL3) – Cy3
Purified CSL3 is conjugated to cyanine 3 (Cy3) fluorescent dye. Cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media, and are brighter in a non-polar environment than in an aqueous medium. Advantages of plastic over aqueous mounting media are brightness, contrast, and longevity of the fluorescence. Advantage of Cy3 conjugates are bright and photostable in the aqueous environment as well as in non-polar media. Cy3 has an approximate peak excitation wavelength of 550 nm and an emission wavelength at 570 nm. This product comes in a stabilized liquid form.
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Hippeastrum hybrid Lectin (HHL) – Biotinylated
Affinity purified Hippeastrum hybrid lectin (HHL) is consists of four subunits of identical size. It binds only to (α-1,3) and (α-1,6) linked mannosyl units of α-mannose residues. HHL reacts not only with terminal but also with internal α-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked α-mannosyl. HHL also binds to some yeast galactomannans. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Hippeastrum hybrid Lectin (HHL) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.
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Vicia villosa Lectin (VVL/VVA) – Biotinylated
Affinity purified Vicia villosa lectin or agglutinin (VVL/VVA) is a tetramer composed of four subunits. VVL consists of A and B subunits, with a dominant isolectin that appears to be B subunit-rich. A4 isolectin agglutinates specifically A1 erythrocytes. B4 isolectin does not agglutinate A, B, or O-erythrocytes; however, B4 binds the Tn antigen, a molecule expressed on the surface of most tumor cells. A2B2 and similar tetramer isolectins display properties characteristic of both A4 and B4 isolections.Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Vicia villosa Lectin (VVL/VVA) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.
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血小板研究-Jacalin
糖尿病誘導的的血小板功能障礙和糖尿病中血小板聚集可能會造成血管疾病並增加得到阿茲海默症的可能性。一項新的研究調查發現anti-α-galactoside (anti-Gal) 和anti-β-glucoside (ABG) 抗體與AOP1 和 AOP2 的蛋白結合形成三聚體。研究人員利用血小板表面 O-glycoproteins上富含serine和threonine的peptide序列作為配體來了解這些三聚體，並使用Jacalin（能專門辨識O-glycoproteins的一種Lectin）處理血小板，進一步去除三聚體複合物。 |
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| 在裸露的血小板經過Jacalin 的處理，阻止了三聚體與血小板的附著並抑制了隨後的 ADP 所誘導血小板的聚集。這代表著 ADP會因為裸露的血小板表面上有大量的O-glycoproteins使血小板聚集。而 anti-Gal 和 ABG未佔據結合位點的三聚體的可能依賴於 GPIIIb/IIIa（一種較大的O-glycoproteins，是 ADP 介導的血小板活化和聚集的重要中間體）作為血小板上的配體，就像Jacalin 對裸露的血小板結合的那樣， 此數據可能為血小板脆弱性及其對糖尿病患者疾病的研究提供新的方向。 |
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