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Mesenchymal-epithelial transition factor (MET), also known as c-Met or Hepatocyte growth factor receptor (HGFR), is a receptor tyrosine kinase (RTK) that is overexpressed or mutated in various malignancies. Aberrantly active MET triggers tumor growth, angiogenesis, and cancer metastasis. MET activity is also critical to embryogenesis.
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HGF, also known as scatter factor (SF), is the only known ligand of MET. Upon activation, MET engages with a variety of signal transducers at its intracellular multisubstrate docking site to stimulate the Ras/MAPK, JAK/STAT, SRC, PI3K/AKT, Wnt/β-catenin, and other signaling pathways. MET and its associated signaling pathways are clinically important therapeutic targets. A number of small-molecule inhibitors and antibodies targeting MET and HGF are being developed as promising therapeutics for cancer, heart disease, autism, and other diseases. Sino Biological has developed high-quality reagents to support the study of MET signaling.
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c-MET and HGF Proteins
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Sino Biological has developed a panel of high activity c-MET proteins and HGF proteins from various species to help study the biology of MET signaling.
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Human c-MET Protein | 10692-H20B1
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Cynomolgus c-MET Protein | 90304-C08H
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Purity:
> 90% as determined by SDS-PAGE
Activity:
• The specific activity was determined to be 10 nmol/min/mg using MBP as substrate.
• Binding ability with HGF measured by functional ELISA.
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Binding ability measured by ELISA. Immobilized human HGF (Cat: 10463-HNAS) at 10 µg/mL (100 µL/well) can bind canine c-MET. The EC50 is 7 ng/mL.
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• Recommended c-MET and HGF Proteins
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Please visit #Sino Biological to search for these products.
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Catalog #
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Species
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Molecule
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Tag
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Expressed Host
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Activity
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10692-H20B1
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Human
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c-MET
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N-GST & His
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Insect Cells
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Active
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50622-M02H
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Mouse
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c-MET
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C-human IgG1-Fc
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HEK293 Cells
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Active
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50622-M08H
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Mouse
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c-MET
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C-His
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HEK293 Cells
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Active
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70008-D08H
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Canine
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c-MET
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C-His
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HEK293 Cells
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Active
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80004-R02H
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Rat
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c-MET
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C-human IgG1-Fc
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HEK293 Cells
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Active
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90304-C02H
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Cynomolgus, Rhesus
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c-MET
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C-human IgG1-Fc
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HEK293 Cells
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Active
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90304-C08H
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Cynomolgus, Rhesus
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c-MET
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C-His
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HEK293 Cells
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Active
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90304-CCCH
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Cynomolgus, Rhesus
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c-MET
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C-cleavage
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HEK293 Cells
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Active
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10463-HNAS
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Human
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HGF
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Native
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CHO Cells
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Active
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50038-M08H
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Mouse
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HGF
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C-His
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HEK293 Cells
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Active
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50038-MNAH
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Mouse
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HGF
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Native
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HEK293 Cells
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Active
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70063-DNAH
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Canine
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HGF
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Native
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HEK293 Cells
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80429-RNAH
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Rat
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HGF
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Native
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HEK293 Cells
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Active
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90286-CNAH
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Cynomolgus
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HGF
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Native
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HEK293 Cells
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Active
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Anti-c-MET and Anti-HGF Antibodies
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Sino Biological has developed a comprehensive panel of antibodies targeting MET and HGF, which can be used in ELISA, FCM, IHC, WB, as well as neutralization. ELISA kits and ELISA pair sets are also available.
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• More Antibodies of c-MET and HGF
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