HGF/c-Met 軸:一種有前景的癌症治療靶點和生物標誌物

HGF/c-Met 軸:一種有前景的癌症治療靶點和生物標誌物

2021.10.29

 本文轉載至原廠電子報:Sino Biological https://www.sinobiological.com/ 電子報原文

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HGF/c-MET Axis: Key Factor Responsible for Cancer Therapy

Mesenchymal-epithelial transition factor (MET), also known as c-Met or Hepatocyte growth factor receptor (HGFR), is a receptor tyrosine kinase (RTK) that is overexpressed or mutated in various malignancies. Aberrantly active MET triggers tumor growth, angiogenesis, and cancer metastasis. MET activity is also critical to embryogenesis.

HGF, also known as scatter factor (SF), is the only known ligand of MET. Upon activation, MET engages with a variety of signal transducers at its intracellular multisubstrate docking site to stimulate the Ras/MAPK, JAK/STAT, SRC, PI3K/AKT, Wnt/β-catenin, and other signaling pathways. MET and its associated signaling pathways are clinically important therapeutic targets. A number of small-molecule inhibitors and antibodies targeting MET and HGF are being developed as promising therapeutics for cancer, heart disease, autism, and other diseases. Sino Biological has developed high-quality reagents to support the study of MET signaling.

 

c-MET and HGF Proteins

Sino Biological has developed a panel of high activity c-MET proteins and HGF proteins from various species to help study the biology of MET signaling.

Human c-MET Protein | 10692-H20B1

 

Cynomolgus c-MET Protein | 90304-C08H

10692-H20B1

Purity:
> 90% as determined by SDS-PAGE

Activity:
• The specific activity was determined to be 10 nmol/min/mg using MBP as substrate.
• Binding ability with HGF measured by functional ELISA.

90304-C08H

Binding ability measured by ELISA. Immobilized human HGF (Cat: 10463-HNAS) at 10 µg/mL (100 µL/well) can bind canine c-MET. The EC50 is 7 ng/mL.

 

Human HGF Protein | 10463-HNAS

Cynomolgus HGF Protein | 90286-CNAH

10463-HNAS

90286-CNAH

Ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 is typically 0.5-5 ng/mL.

Ability to inhibit TGFβ1 activity on Mv-1-lu mink lung epithelial cells. The ED50 is typically 2-10 ng/mL.

• Recommended c-MET and HGF Proteins

Please visit #Sino Biological to search for these products.

Catalog #

 

Species

Molecule

Tag

Expressed Host

Activity

10692-H20B1

Human

c-MET

N-GST & His

Insect Cells

Active

50622-M02H

Mouse

c-MET

C-human IgG1-Fc

HEK293 Cells

Active

50622-M08H

Mouse

c-MET

C-His

HEK293 Cells

Active

70008-D08H

Canine

c-MET

C-His

HEK293 Cells

Active

80004-R02H

Rat

c-MET

C-human IgG1-Fc

HEK293 Cells

Active

90304-C02H

Cynomolgus, Rhesus

c-MET

C-human IgG1-Fc

HEK293 Cells

Active

90304-C08H

Cynomolgus, Rhesus

c-MET

C-His

HEK293 Cells

Active

90304-CCCH

Cynomolgus, Rhesus

c-MET

C-cleavage

HEK293 Cells

Active

10463-HNAS

Human

HGF

Native

CHO Cells

Active

50038-M08H

Mouse

HGF

C-His

HEK293 Cells

Active

50038-MNAH

Mouse

HGF

Native

HEK293 Cells

Active

70063-DNAH

Canine

HGF

Native

HEK293 Cells

 

80429-RNAH

Rat

HGF

Native

HEK293 Cells

Active

90286-CNAH

Cynomolgus

HGF

Native

HEK293 Cells

Active

 

Anti-c-MET and Anti-HGF Antibodies

Sino Biological has developed a comprehensive panel of antibodies targeting MET and HGF, which can be used in ELISA, FCM, IHC, WB, as well as neutralization. ELISA kits and ELISA pair sets are also available.

Cell Proliferation was Inhibited by Human HGF Antibody | 10463-mh010

Flow Cytometric Analysis of Human c-Met Expression on HepG2 Cells | 10692-R243

10463-mh010

10692-R243

Proliferation of U87 MG cells elicited by autocrine HGF was inhibited by increasing concentrations of Human HGF Neutralizing Monoclonal Antibody. The IC50 is typically 1-4 µg/mL.

Cells were stained with purified anti-Human c-Met, second antibody conjugated with FITC.

• More Antibodies of c-MET and HGF

Anti-c-MET Antibodies

 

Anti-HGF Antibodies

Sino Biological

Sino Biological US Inc.
Tel:+1-215-583-7898
Order:order_us@sinobiologicalus.com
CRO Service:cro_us@sinobiologicalus.com
Address1400 Liberty Ridge Drive, Suite 101, Wayne, PA 19087

 

 

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