|
|
|
MCE MiRNAs Related Products
MedChemExpress (MCE) offers a range of miRNA mimics, inhibitors, agomirs, and antagomirs based on mature miRNA sequences from human, mouse, and rat miRBase database. These products are designed to facilitate the study of miRNA function in a variety of research applications.
Unlock the power of 18,000+ miRNAs from our extensive collection at MedChemExpress! Whether you’re working on cellular experiments or in vivo applications, our miRNA tools are designed to help you advance your research with precision. |
|
|
|
miRNA mimics: Resemble endogenous miRNA duplex sequences. Mimicking natural miRNA activity.
miRNA inhibitors: Specifically binds to mature miRNA and inhibits miRNA function. Enabling precise control over miRNA-related gene regulation.
miRNA agomirs: Resemble endogenous miRNA duplex sequences. Mimicking natural miRNA activity.
miRNA antagomirs: Specifically binds to mature miRNA and inhibits miRNA function. Enabling precise control over miRNA-related gene regulation. |
|
|
|
Victor Ambros and Gary Ruvkun have been jointly awarded the 2024 Nobel Prize in Physiology or Medicine for their groundbreaking discovery of microRNA (miRNA) and its role in post-transcriptional gene regulation. Their research has fundamentally changed our understanding of gene expression, shedding light on how small RNA molecules regulate the transcription and translation of genes into proteins, with significant implications for developmental biology and diseases.
|
|
|
|
What is MiRNA？
MiRNAs are small, endogenous non-coding RNAs (21-23 nt) that bind to specific target messenger RNA (mRNA) to modulate their expression [2]. First identified by Victor Ambros and colleagues in Caenorhabditis elegans in 1993, miRNAs regulate gene expression through a post-transcriptional mechanism. The team discovered that lin-4 RNAs suppress the translation of the lin-14 gene via an antisense interaction [1]. Since this discovery, thousands of miRNAs have been identified across a wide range of organisms, including humans, mice, and plants. MiRbase (https://www.mirbase.org/) provides a curated collection of published miRNA sequences and their associated biological data. |
|
|
|
The Biogenesis of MiRNA
MiRNA biogenesis begins with transcription by RNA polymerase II (Pol II), producing a long primary miRNA (pri-miRNA) with more than 1,000 nt. This pri-miRNA is then processed by Drosha, a type III RNase, along with its cofactor DGCR8, into a hairpin-structured pre-miRNA (~70 nt)[3]. Exported to the cytoplasm via Exportin 5, the pre-miRNA is further cleaved by Dicer into a miRNA duplex (~22 nt). Argonaute (Ago) proteins then unwind the duplex, forming the RNA-induced silencing complex (RISC), with one strand preferentially loaded to carry out gene silencing [1]. |
|
|
|
Figure 1. miRNA biogenesis[4]
|
|
|
|
Mechanism of Action
MiRNAs typically bind to 3′-UTR of target mRNA. For mammalian cells, imperfect base-pairing leads to suppression of mRNA translation, while in plant cells, near-perfect complementarity results in mRNA degradation. Notably, miRNAs can target multiple genes thanks to the flexibility of their base pairing [5,6]. |
|
|
|
[1]. Theranostics. 2021;11(18):8771-8796.
[2]. Gene Ther. 2011;18(12):1104-1110.
[3]. Mol Cancer. 2018;17(1):64.
[4]. Nat Rev Drug Discov. 2017;16(3):203-222.
[5]. Mol Cancer. 2021;20(1):54.
[6]. Cells. 2020;9(1):137.
|
|
|
|
|
|
|
|
Products are for research use only and are not intended for human use. We do not sell to patients.
|
|
|
|
|
