Research Area: P2X7 Receptor Antagonist/Neuroinflammation and CNS Diseases
Orally active, rodent-active, and CNS-penetrant P2X7 receptor antagonist (IC50s=12 and 2.4 nM for human and rat P2X7 receptor, respectively; Kis=22, 54, and 13 nM for mouse, human and rat P2X7 receptor, respectively).
Has the potential for the research of CNS disease.
Solubility: DMSO : 125 mg/mL (251.04 mM; Need ultrasonic)
MCE Annexin V-FITC/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis.
After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).
MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.
The content of DNA is changed with the process of cell cycle. DNA can be stained by fluorescent dye, such as Propidium Iodide (PI), to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1/S/G2/M phase as well as apoptosis cells with signals at sub-G1 region.
Markedly inhibits TGF-β1-mediated migration and invasion by deactivating epithelial-mesenchymal transition (EMT) via the TGF-β/Smad and PI3K/Akt/mTOR pathways in HCC cells. Exhibits no cytotoxic effects on normal liver LO2 cells.
Possesses potent anti-inflammatory effects in LPS-induced macrophage cell line mediated by inhibition of release of inflammatory mediators, NO, PGE2, and pro-inflammatory cytokines.
Significantly induces reduction in the mRNA expressions of inducible nitric oxide synthase and cyclooxygenase-2.