OZ Biosciences | 全新推出：優質的自我擴增 RNA (self-amplifying RNA, saRNA) 產品

OVA, HA H1N1 and Spike Sars Cov-2 are now available as self-amplifying RNA  !

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We are pleased to announce that our saRNA (Self-amplifiying RNA) range is expanding : OVA, HA H1N1 and Spike Sars Cov-2 saRNAs join GFP and LUC saRNAs.

OZB saRNA have been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase.

Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.

saRNAs have been designed to produce high expression level of protein with enhanced length of duration. OZB mRNAs are produced by in vitro transcription. mRNAs are stabilized at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end. Sequences have been optimized to yield improved stability and performance.

Fig 1 : GFP protein expression over time in HEK293 cells in 24-well plate cells upon lipid-based transfection with either 0,4 µg/well of Replicon GFP or 0,4 µg/well of classic mRNA GFP using NL51 transfection reagent (ratio 2:1). Photos were taken at x20 show a peak of GFP expression at 24hic mRNA GFP. 

New enzyme for low dsRNA in our mRNAs !

As part of our continuous quality improvement, we have selected a new generation of RNA polymerase that reduces dsRNA during IVT. We are able to reduce the amount of dsRNA in our catalog and in our customized mRNAs by up to 88%.

Our new quality control limit is 0.05% of dsRNA.

Fig 2 : Comparison of dsRNA amount in different mRNA produced by OZ Biosciences with the old  the new RNA Polymerase.

OVA saRNA is available in two ways :

• #MRNA74 does not bear any additional nucleotide modifications.
• #MRNA75 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).

HA H1N1 saRNA is available in two ways :

• #MRNA76 does not bear any additional nucleotide modifications.
• #MRNA77 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).

Spike Sars-Cov-2 saRNA is available in two ways :

• #MRNA78 does not bear any additional nucleotide modifications.
• #MRNA79 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).

GFP saRNA is available in two ways:

• #MRNA70 does not bear any additional nucleotide modifications.
• #MRNA71 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).

LUC saRNA is available in two ways :

• #MRNA72 does not bear any additional nucleotide modifications.
• #MRNA73 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine).

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