Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A or NB2B subunit of the NMDAR respectively phosphorylated. Lanes 1/2 are labeled with NR2A Tyr1325 and Lanes 3/4 are labeled with NR2B Tyr1472. Phosphospecificity is shown in the second and fourth lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
This summer has been a sizzler!! Have you ever wondered what happens to the brain during a heatstroke? Check out this article from Brain Facts describing how our body regulates the extreme heat of summer when outdoors.