Accutase® 細胞分離(Cell Detachment)試劑
AT-104
100 mL，500 mL
Brand
Innovative Cell Technologies
Description
Accutase® 是一種即用型非哺乳動物、非細菌替代品，適用於胰蛋白酶的所有應用。 Accutase的優勢: •Can be used whenever gentle and efficient detachment of any adherent cell line is needed. Accutase is a direct replacement for trypsin. •Works extremely well on embryonic and neuronal stem cells; mono layers of stem cells can be grown after passaging with Accutase. •Preserves most epitopes for subsequent flow cytometry analysis. •Does not need to be neutralized when passaging adherent cells. The addition of more media after the cells are split dilutes Accutase so it is no longer able to detach cells. •Does not need to be aliquoted. A bottle is stable in the refrigerator for 2 months. Cell Lines tested: A few cell lines that Accutase has been shown to detach without harm: hESCs, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Application
Reactivity
