Activated Protein C (APC), human

Brand

ARP

Description

pDESCRIPTION: Recombinant human Activated Protein C (rHuAPC)br /
Activated Protein C exerts an antithrombotic effect by inhibiting Factors Va and VIIIa. In vitro data indicate that Activated Protein C has indirect profibrinolytic activity through its ability to inhibit plasminogen activator inhibitor-1 (PAI-1) and limiting generation of activated thrombin-activatable-fibrinolysis-inhibitor. Additionally, in vitro data indicate that Activated Protein C may exert an anti-inflammatory effect by inhibiting human tumor necrosis factor production by monocytes, by blocking leukocyte adhesion to selectins, and by limiting the thrombin-induced inflammatory responses within the microvascular endothelium.br /
rHuAPC is a disulfide-linked homodimeric, glycosylated, Polypeptide chain having a molecular mass of 55kDa. It is a serine protease with the same amino acid sequence and glycosilation sites as the human plasma-derived Activated Protein Cbr /
SOURCE: Chinese Hamster Ovary Cellsbr /
PURITY: gt; 99%, as determined by:br /
a) Analysis by RP-HPLCbr /
b) Anion-exchange FPLCbr /
c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gelbr /
ENDOTOXIN: lt; 0.1 ng/mu;g (IEU/mu;g) of APCbr /
DIMERS AND AGGREGATES: lt; 1% as determined by silver-stained SDS-PAGE gel analysisbr /
PROTEIN CONTENT: Protein quantitation was carried out by two independent methods:br /
1) UV spectroscopy at 280 nmbr /
2) Analysis by RP-HPLC, using a standard solution of APC as a Referencebr /
Standardbr /
FORM: Purified, in 7.6mg sodium chloride, 1.5mg citrate and 6mg sucrosebr /
STORAGE: -20deg;C (aliquot), avoid freezing and thawing cycles/p

Application

Reactivity

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