Anti-Aquaporin 3
AQP-003
50 μl 0.2ml
Brand
Description
Anti-Aquaporin 3
| Product#: | AQP-003 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
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Rabbit.
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| Epitope: |
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Peptide (C)STEAENVKLAHMKHKEQI, corresponding to residues 275-292 of rat AQP3 (Accession P47862).
|
 |
| Putative epitope location: |
|
Intracellular, C-terminus.
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| Homology: |
|
Mouse – 17/18 residues identical; sheep, human – 15/18 residues identical.
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| Reactivity Confirmed: |
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Rat, mouse.
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Applications:
| Western Blotting: |
 |
Western blotting of rat kidney membranes:
1. Anti-Aquaporin 3 antibody (#AQP-003) (1:200).
2. Anti-Aquaporin 3, preincubated with the control peptide antigen.
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| Immunohistochemistry: |
 |
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Expression of Aquaporin 3 in rat colon.
Paraffin embedded longitudinal section of rat colon showing mucosa (M), submucosa (SM) and muscularis externa (ME). Staining with Anti-Aquaporin 3 antibody (#AQP-003) (brown color) is highly specific for absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Counterstain is Hematoxilin.
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| References: |
| 1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. |
| 2. Hara-Chikuma, M. et al. (2003) Proc. Natl. Acad. Sci. USA. 100, 7360. |
| 3. Hara-Chikuma, M. and Verkman, A.S. (2008) Mol. Cell. Biol. 28, 326. |
Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7, AQP-9 and AQP-10.1
Little is known about the function of the two newest members, AQP-11 and AQP-12.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1
AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.2 Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of the rat Aquaporin 3 channel. Anti- Aquaporin 3 antibody (#AQP-003) can be used in Western blot and immunohistochemical applications, and recognizes the Aquaporin 3 channel from rat and mouse samples.
Application
Reactivity
