Anti-ENaCg
Description
Anti-ENaCg
(Epithelial Na+ channel g subunit, Amiloride-sensitive Na+ channel g subunit, SCNN1G)
The amiloride-sensitive epithelial Na+ channel (ENaCs) family includes 4 members:
ENaCa, b, g and d. The ENaC channel is located in the luminal (apical) plasma membrane of several epithelial tissues such as kidney, lung, salivary glands and skin.
The functional channel is believed to be a multimer including a a (or d) and a b and g subunits with a likely stoichiometry of a2bg. The ENaC channel enables entry of Na+ into the cell along its electrochemical gradient and thus has a central role in the maintenance of renal Na+ balance (and hence blood pressure) and liquid balance in the lung.
Indeed, genetic mutations in the ENaC subunits causes Liddleî–¸ syndrome (a form of hypertension)
or pseudohypoaldosteronism type 1 (PHA) that is characterized by hypotension.
The ENaC channel is voltage-independent and is constitutively active in epithelia although it is modulated by several different mechanisms.
One of the main mechanisms is the controlled internalization of the channel that is dependent on the b or g subunits. Indeed, mutations in the C-termini of these subunits reduce endocytosis of the channel leading to the accumulation of ENaC in the cell membrane and causing a phenotype that is consistent with that of Liddleî–¸ syndrome.
| Epitope: |
| Peptide (C)YGVKESRKRREAGS, corresponding to amino acid residues 129-142 of rat ENaCg (Accession P37091). |
| Putative epitope location: |
| Extracellular. |
| Homology: |
| Human-13/17 residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
| Rat lung lysate (1:200). |
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Western blotting of rat lung lysate:
1. Anti- ENaCg antibody (1:200)
2. Anti- ENaCg antibody, preincubated with the control peptide antigen.
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Control antigen included in price
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1.
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Kellenberger, S. et al. (2003) J. Biol.Chem. 278, 29153.
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2.
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Snyder, P.M. (2002) Endocrine Rev. 23, 258. |
