Anti-Proteasome 20S Subunit α5

PDF

AP-120

100 μg

Brand

Boston Biochem

Description

Anti-Proteasome 20S Subunit α5

Catalog #: AP-120

Anti-Proteasome 20S Subunit α5
Product Source: 

rabbit polyclonal

The proteasome is a large, multimeric protease that catalyzes the final step of the UPP intracellular protein degradation. The proteasome exists in multiple forms within the eukaryotic cell, and contained in all isoforms is the catalytic core known as the 20S proteasome. The 700kDa 20S proteasome is arranged as four axially stacked heptameric rings with multicatalytic centers located within the internal cavity of the ß-subunits. The proteasome’s endogenous substrates are polyubiquitinated proteins. The form of the proteasome that recognizes and degrades these proteins is the 26S proteasome. This 1500 kDa species is formed in an ATP dependent manner with the addition of two copies of the 19S regulator on the 20S catalytic core. The 19S regulator complex is composed of 16 different subunits ranging in size from 30 kDa to 120 kDa and contains an ubiquitin chain receptor, ATPase activity and deubiquitinating activity.

Product Information
Quantity: 

100 μg

Stock: 

100 μg of IgG in PBS with 1 mg/ml BSA + 0.05% NaN3. Epitope affinity purified.

Immunogen: 

Synthetic Peptide: CA120ILQFGEEDADPGAM133 from mouse proteasome 20S subunit α5. The sequence is 94% conserved in human and rat.

Reactivity: 

Antibody detects proteasome 20S α5 subunit from human, hamster, mouse and rat (26.5 kDa).

 

Use & Storage
Storage: 

Store vial at -80°C. Avoid repeated freeze-thaw cycles.

Specificity: 

Recommended dilution for Western blot is between 1:100 and 1:300. Investigator should empirically determine optimal working dilutions.

Application

Reactivity

Photos

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