ApoBrdU-IHC DNA Fragmentation Assay Kit

Alternate Name :

Tag Line :

Two-Color TUNEL Assay

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Species reactivity- Mammalian
• Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation

Detection Method:

Features & Benefits:

• Simple procedure; convenient
• The assay is sensitive and stable
• The assay offers an one-step labeling of apoptotic cells.

Description:

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H₂O₂/Urea tablets for color generation and methyl green solution for counter staining the cells.