Beta-Mannosidase Activity Assay Kit (Fluorometric)

Alternate Name : Beta-Mannosidase Activity Assay,Beta-Mannosidase Activity Assay Kit,EC 3.2.1.25,Mannosidase Activity Assay,b-Mannosidase Activity Assay Kit,Beta-Mannosidase Activity Kit,β-Man,Mannosidase Activity Assay Kit,Beta-Mannosidase Activity Assay (Fluorometric),Beta-Mannosidase Activity Kit (Fluorometric),b-Mannosidase Activity Assay Kit (Fluorometric),Beta-Mannosidase Activity Assay Kit (Fluorometric)

Tag Line :

To monitor total β-Mannosidase activity in various biological samples

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

Beta-mannosidase (β-Man; EC 3.2.1.25) is an enzyme that hydrolyzes terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. It is a member of the glycoside hydrolase family 2. There are numerous applications of β-Man including the synthesis of glycosides, production of fermentable sugars, curing mannosidosis, etc. Lysosomal β-Man is essential for catabolizing the Asn-linked glycans of glycoproteins thereby maintaining the cellular homeostasis. β-Man deficiency results in Beta-Mannosidosis, a rare inherited disorder affecting the break-down of mannose-containing disaccharides. These disaccharides gradually accumulate in the lysosomes and cause cells to malfunction. BioVision’s Beta-Mannosidase Activity Assay Kit provides a facile, rapid way to monitor total β-Man activity in various biological samples. In this kit, β-Man cleaves a synthetic specific substrate and releases a fluorophore, which can be easily quantified (Ex/Em = 360/445 nm). The substrate is specific to β-Mannosidase and can differentiate from α-Mannosidase. The assay is simple, sensitive and can detect as low as 1.0 µU of beta-mannosidase activity.

Detection Method: Fluorescence detection Ex/Em = 360/445 nm

Features & Benefits:

Can detect as low as 1.0 µU of beta mannosidase activity.,The substrate is specific to β-Man and can differentiate from β-mannosidase,A facile, rapid way to monitor total Beta-Mannosidase in various biological samples.

Description:

Beta-mannosidase (β-Man; EC 3.2.1.25) is an enzyme that hydrolyzes terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. It is a member of the glycoside hydrolase family 2. There are numerous applications of β-Man including the synthesis of glycosides, production of fermentable sugars, curing mannosidosis, etc. Lysosomal β-Man is essential for catabolizing the Asn-linked glycans of glycoproteins thereby maintaining the cellular homeostasis. β-Man deficiency results in Beta-Mannosidosis, a rare inherited disorder affecting the break-down of mannose-containing disaccharides. These disaccharides gradually accumulate in the lysosomes and cause cells to malfunction. BioVision’s Beta-Mannosidase Activity Assay Kit provides a facile, rapid way to monitor total β-Man activity in various biological samples. In this kit, β-Man cleaves a synthetic specific substrate and releases a fluorophore, which can be easily quantified (Ex/Em = 360/445 nm). The substrate is specific to β-Mannosidase and can differentiate from α-Mannosidase. The assay is simple, sensitive and can detect as low as 1.0 µU of beta-mannosidase activity.