Bovine A1 Beta Casein ELISA Kit (1 plate)

ZZZZ_Status (Active, Disc) : Active

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

Beta Casein; CSN2

Antibody_Isotype
(Antibody only) :

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

Background_Info : The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.

According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow’s milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.

A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.
A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478). .
Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.

Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.

Clone_Name
(AB only, monoclonals) :

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1665

Description (Long Description) : 

Bovine milk may contain two types of beta-casein protein, A2 or A1, which differ by a single-point mutation at amino acid position 82 (P82/H82). Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes. The Biosensis Bovine A1 Beta Casein ELISA Kit is a sandwich ELISA that allows the specific quantification of the A1 isoform of bovine beta casein in bovine milk, only if used as directed. The A2 isoform is not detected in this ELISA assay. Please refer to the kit protocol for specific use instructions.

Expiry_Date : 

6 months from purchase

Format (Form) : 

Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

Immunogen (Antigen) :Standard is purified bovine A1

Kit_Components : ELISA plate pre-coated with rabbit anti-pan casein antibody, 1 x 96-well microplate, store at 2-8C
BSA for antibody dilution buffer, lyophilized, store at 2-8C
10X TBS, store at 2-8C
Tween-20, 50%, 1 mL, store at 2-8C
Native A1 beta casein protein standard, 40 ug/mL, store at -20C
Chicken anti-A1 beta casein detection antibody, 0.5 mg/mL, store at -20C
Rabbit anti-chicken HRP conjugate, store at -20C
TMB substrate, store at 2-8C

MSDS (File name) :