Canine TIGIT Protein, His Tag

Source : 

Canine TIGIT, His Tag (TIT-C52H7) is expressed from human 293 cells (HEK293). It contains AA Phe 17 – Pro 208 (Accession # F1PKJ0-1).

Molecule : TIGIT

Synonyms : TIGIT,VSIG9,VSTM3

Format : Powder

Category : Immune Checkpoint Proteins

Accession : N/A

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : 23.1 kDa

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.1 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : PBS, pH7.4

Description :

TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells(NK). It is also identified as WUCAM and Vstm3. TIGIT could bind to CD155(PVR) on dendritic cells(DCs), macrophages, etc. with high affinity, and also to CD112(PVRL2) with lower affinity. TIGIT expressing CD8+ T cells has been shown to be expanded and associated with clinical markers of HIV disease progression in a diverse group of HIV infected individuals. TIGIT and PD-1 has been shown to be over expressed on tumor antigen-sepcific (TA-specific) CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of TA-specific CD8+ T cells and TIL CD8+ T cells.

References :

(1)Chew GM., et al., 2016, PLoS Pathogens., 12: e1005349.
(2)Joe-Marc Chauvin, et al., 2015, J Clin Invest., 125 (5): 2046-2058.
(3)Steven G. Deeks, 2012, Nature, 487 (1): 439-440.