CLEC-2 Protein, Human, Recombinant (His Tag)

CLEC-2 Protein, Human, Recombinant (His Tag): Product Information

Purity

> 76 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

1. Measured by its binding ability in a functional ELISA.

2. Immobilized human Podoplanin (Cat: 10012-H08H) at 10 μg/mL (100 μl/well) can bind  biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.71 μg/mL.

Protein Construction

A DNA sequence encoding the human CLEC1B (NP_057593.3) extracellular domain (Gln 58-Pro 229) with a N-terminal polyhistidine tag was expressed.

Accession#

NP_057593.3

Expressed Host

HEK293 Cells

Species

Human

Predicted N Terminal

His

Molecule Mass

The recombinant human CLEC1B comprises 188 amino acids with a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh CLEC1B is approximately 35-38 kDa in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4

Please contact us for any concerns or special requirements.

Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.