ErbB4 cDNA ORF Clone, Human, C-Myc tag

ErbB4 cDNA ORF Clone, Human, C-Myc tag: General Information

Gene

Species

Human

NCBI Ref Seq

NM_001042599.1

RefSeq ORF Size

3924 bp

Sequence Description

Identical with the Gene Bank Ref. ID sequence.

Description

Full length Clone DNA of Human v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian), transcript variant JM-a/CVT-2 with C terminal Myc tag.

Plasmid

Promoter

Enhanced CMV promoter

Vector

pCMV3-C-Myc

Restriction Sites

KpnI(two restriction sites) + XbaI(6kb+2.22kb+1.72kb)

Tag Sequence

Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG

Sequencing Primers

T7( 5′ TAATACGACTCACTATAGGG 3′ )

BGH( 5′ TAGAAGGCACAGTCGAGG 3′ )

Quality Control

The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli

Kanamycin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression