KitsFerret CD4 ELISA Pair Set

PDF

SEK60003

Brand

Sino Biological

Description

分子 : CD4

 

保存條件:

Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

 

 

線性範圍 : 46.88-3000

 

包被抗體 : 1 mg/mL of mouse anti-Ferret CD4 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 60003-MM08)

 

檢測抗體: 0.2 mg/mL of mouse anti-Ferret CD4 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 60003-MM02)

 

標準品 : Each vial contains 140 ng of recombinant Ferret CD4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.

 

生物素交聯HRP:

 

靈敏度 : The minimum detectable dose of Ferret CD4 was determined to be approximately 46.88 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

特異性 :

 

檢測原理 : The Ferret CD4 ELISA Pair Set is for the quantitative determination of Ferret CD4.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Ferret CD4 coated on a 96-well plate. Standards and samples are added to the wells, and any Ferret CD4 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Ferret CD4 monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Ferret CD4 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

 

檢測限: 46.88 pg/ml

 

血清樣本檢測範圍 :

 

Application

Reactivity

Photos