G protein alpha antibody

Product Name 

G protein alpha antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to G protein alpha

Immunogen 

Synthetic peptide: RMHLRQYELL conjugated to KLH, corresponding to C terminal amino acids 385-394 of Human G protein alpha.
BLAST ‘RMHLRQYELL’

Mamm

Specificity 

ab54490 does not cross-react with G_i alpha-1, G_i alpha-2, G_i alpha-3, or G_o alpha. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for G_s alpha, G_i alpha-1, G_i alpha-2, G_i alpha-3, and G_o alpha.

WB

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 46 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane

Relevance 

GTP-binding proteins (G-proteins)are a family of heterotrimeric proteins that play a critical role in signal transduction by coupling cell surface, 7-transmembrane domain receptors to intracellular signaling pathways including second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth and differentiation. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 140mM Sodium chloride, 100mM Potassium phosphate, pH 7.5

Form 

Liquid

Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.