Goat anti-FOXP3 / SCURFIN Antibody
G-1912-100
100 μg
Brand
biosensis
Description
ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
FOXP3; SCURFIN; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; JM2 protein; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; FOXP3delta7; scurfin
Antibody_Isotype
(Antibody only) :
Application_Details :
Western Blot: Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.
Peptide ELISA: antibody detection limit dilution 1:8000.
Background_Info :
Clone_Name
(AB only, monoclonals) :
Datasheet_Link :
Description (Long Description) :
Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (Uniprot).
Expiry_Date :
12 months after purchase if unopened.
Format (Form) :
Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Immunogen (Antigen) :A synthetic peptide consisting of amino acids, SQRPSRCSNPTPGP
Kit_Components :
MSDS (File name) :
Application
Pep-ELISA, WB, IF, FC, ICC
Reactivity
Humanother species not yet tested