ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
FOXP3; SCURFIN; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; JM2 protein; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; FOXP3delta7; scurfin
(Antibody only) :
Western Blot: Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.
Peptide ELISA: antibody detection limit dilution 1:8000.
(AB only, monoclonals) :
Description (Long Description) :
Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (Uniprot).
12 months after purchase if unopened.
Format (Form) :
Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Immunogen (Antigen) :A synthetic peptide consisting of amino acids, SQRPSRCSNPTPGP
MSDS (File name) :