Goat anti-PPP1R15A / GADD34 Antibody
G-1864-100
100 μg
Brand
biosensis
Description
ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
PPP1R15A; GADD34; protein phosphatase 1, regulatory (inhibitor) subunit 15A; growth arrest and DNA-damage-inducible 34; protein phosphatase 1, regulatory subunit 15A
Antibody_Isotype
(Antibody only) :
Application_Details :
Western Blot: Approx 75kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: KLY (Kelly Human neuroblastoma) cell lysate.Immunohistochemistry: In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration: 2-4µg/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Peptide ELISA: antibody detection limit dilution 1:128000.
Background_Info :
Clone_Name
(AB only, monoclonals) :
Datasheet_Link :
Description (Long Description) :
Acts as a transcriptional regulator that recognizes and binds to the sequence 5′-[GA]TTA[CT]GTAA[CT]-3′, a sequence present in many cellular and viral promoters (Uniprot).
Expiry_Date :
12 months after purchase if unopened.
Format (Form) :
Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-AAALDLSGRRG
Kit_Components :
MSDS (File name) :
Application
Pep-ELISA, WB, IHC, IF, FC
Reactivity
Humanother species not yet tested