Goat anti-TMP21 / TMED10 Antibody

ZZZZ_Status (Active, Disc) : Active

AA_Supplier Name : Biosensis

Alternative_Name (Other Names) :

TMED10; transmembrane emp24-like trafficking protein 10 (yeast); P24(DELTA); S31I125; S31III125; TMP21; Tmp-21-I ; 21 kDa transmembrane trafficking protein; transmembrane emp24 domain-containing protein 10

Antibody_Isotype
(Antibody only) :

Application_Details :

Western Blot: Preliminary experiments gave an approx 75kDa band in Rat Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_006818.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.Immunofluorescence:Expression of the protein seen in the Golgi and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Peptide ELISA: antibody detection limit dilution 1:1000.

Background_Info :

Clone_Name
(AB only, monoclonals) :

Datasheet_Link :

Description (Long Description) : 

Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side Uniprot).

Expiry_Date : 

12 months after purchase if unopened.

Format (Form) : 

Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-YLRRFFKAKKLIE

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MSDS (File name) :