GST-NEDD8 Activating Enzyme (E1)

PDF

E-312

50 μg

Brand

Boston Biochem

Description

GST-NEDD8 Activating Enzyme (E1)

Catalog #: E-312

GST-NEDD8 Activating Enzyme (E1)
Product Source: 

human recombinant

Also know as: GST-NEDD8 Activating Enzyme (APPBP1/Uba3)

The ATP-coupled activation of NEDD8 that is required for subsequent charging of the NEDD8-specific E2 UbcH12 is catalyzed by heterodimeric APPBP1-Uba3 in humans. The enzyme catalyzes the activation of the C-terminal carboxyl group of NEDD8 by forming a high-energy thioester bond in an ATP-dependent manner.    Uba3 shows 43% homology to the C-terminal half of the ubiquitin activating E1 enzyme Uba1. The Uba3- catalyzed activation of NEDD8 exhibits an absolute requirement for APPBP1 which has high homology to the N-terminal half of Uba1.

Product Information
Quantity: 

50 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl. Actual concentration will vary with specific lot number.

MW: 

135 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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