GST-SUMO Activating Enzyme (E1)

PDF

E-310

50 Î¼g

Brand

Boston Biochem

Description

GST-SUMO Activating Enzyme (E1)

Catalog #: E-310

Product Source:

S. cerevisiae

Also know as: GST-SUMO Activating Enzyme (Aos1/Uba2)

Conjugation of the ubiquitin-like modifier SUMO requires the activities of the heterodimeric E1 (Aos1/Uba2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1 (also SUMO-2 and SUMO-3), forming a high-energy thiolester bond with the cysteine residue of Uba2 and the release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO-1 is transferred to Cys⁹³ of UbcH9.

Product Information

Quantity:

50 μg

Stock:

X mg/ml (X μM) in 50 mM HEPES pH8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.

MW:

140 kDa

Purity:

> 90% by SDS-PAGE

Use & Storage

Use:

Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.

Application

Reactivity

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GST-SUMO Activating Enzyme (E1)