HA-Ubiquitin

PDF

U-110

1 mg

Brand

Boston Biochem

Description

HA-Ubiquitin

Catalog #: U-110

HA-Ubiquitin
Product Source: 

human recombinant

This fully functional N-terminal HA-tagged ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The HA peptide sequence (YPYDVPDYA) is an epitope derived from the influenza hemagglutinin protein. This tag is specifically recognized by anti-HA antibodies and anti-HA-agarose. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosomeindependent events such as protein localization, activity and function.

Product Information
Quantity: 

1 mg

MW: 

9.4 kDa

Purity: 

> 95% by SDS-PAGE

Solubility: 

Aqueous solutions up to 10 mg/ml

Supplied: 

This is a lyophilized powder

 

Use & Storage
Use: 

Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions

Storage: 

Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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