His-tag Protein ELISA kit

Alternate Name :

Tag Line :

A competitve ELISA kit for the quantitative measurement of Bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies

Detection Method: Absorbance (450 nm)

Features & Benefits:

This ELISA kit is used for in vitro quantitative determination of His-Tag Protein
Detection Range: 25.6 – 3200 ng/ml
Sensitivity: 20 ng/ml

Description:

A His-tag is an amino acid motif that contains at least six consecutive histidine (His) residues, often at the N- or C-terminus of a protein. More than 50% of recombinant proteins expressed by eukaryotic or prokaryotic expression systems are tagged with His-tag. Selective, sensitive and quantitative detection of protein levels and protein-protein binding partners expressed with His-tag is therefore a valuable tool in Life Science research. BioVision’s His-Tag Protein ELISA Kit is a competitive-based ELISA that is easier, faster and more sensitive method to detect His-tag proteins expressed in both bacterial, insect and mammalian cells. This detection kit offers ready-to-use reagents, and can detect as low as 20 ng/ml of His-tag protein within 90 min.