His6-hHR6A

PDF

E2-612

50 μg 100 μg

Brand

Boston Biochem

Description

His6-hHR6A

Catalog #: E2-612

His6-hHR6A
Product Source: 

human recombinant

Also known as: His6-hHR6A (human Homolog of RAD6A)

The human HR6A and HR6B proteins share about 95% amino acid sequence identity with each other and about 70% with yeast counterparts, but notably lack the acidic C-terminal domain found in S. cerevisiae proteins. The RAD6 pathway is essential to post-replication repair of DNA and damage-induced mutagenesis in eukaryotic cells. hHR6 protein expression is cell cycle regulated and function with RAD5 and RAD18 RING-finger proteins. The human proteins function similar to the yeast homologs, and may have additional roles in chromatin remodeling and spermatogenesis Residue Ser120 is an important regulatory site in hHR6A, being phosphorylated in vitro by CDK1/2 on which increases ubiquitin conjugating activity. A nonsense mutation in hHR6A has been linked to a novel X-linked mental retardation syndrome (XMLR). This protein has an N-terminal His6 tag, Accession NP_003327.

Product Information
Quantity: 

50 μg 100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration will vary with lot number.

MW: 

20 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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