His6-UbcH3, Dominant Negative

PDF

E2-611

100 μg

Brand

Boston Biochem

Description

His6-UbcH3, Dominant Negative

Catalog #: E2-611

His6-UbcH3, Dominant Negative
Product Source: 

human recombinant

UbcH3 plays an essential role in the progression of cells from the G1 to S phase of the cell division cycle. One pathway (requiring Cdc34) initiates DNA replication by degrading a CDK (cyclin-dependent kinase) inhibitor. The second pathway, involves the anaphase-promoting complex (APC) initiates chromosome segregation and exit from mitosis by degrading anaphase inhibitors and mitotic cyclins. The active site of this enzyme has been chemically inactivated for use as a negative or competitive control.

Product Information
Quantity: 

100 μg

Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.

MW: 

27 kDa

Purity: 

> 95% by SDS-PAGE

 

Use & Storage
Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Application

Reactivity

Photos

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