HRV-3C Protease Cleavage Enzyme, GST Tag, His Tag

Source : 

HRV-3C Protease Cleavage Enzyme, GST Tag, His Tag is a recombinant form of human rhinovirus (HRV) type 14 3C protease (22 KDa on SDS-PAGE) produced in Escherichia coli cells at ACRObiosystems.

Molecule : 3C (HRV)

Synonyms : HRV-3C Protease,3C Protease

Format : Powder

Category : Enzymes

Accession : N/A

Storage : -20℃

Shipping condition : Powder,RT

Molecular Weight : N/A

Characteristics :

HRV-3C Protease Cleavage Enzyme, GST Tag, His Tag is a recombinant form of human rhinovirus (HRV) type 14 3C protease (22 KDa on SDS-PAGE) produced in Escherichia coli cells at ACRObiosystems.

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

Buffer : 50 mM Tris, 150 mM NaCl, 1 mM EDTA, 0.05% Tween20

Description :

Protease Recombinant is fusion protein of GST and human rhinovirus (HRV) type 14 3C protease.Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃, even retaining robust activity at 4℃. Thus, cleavage can be performed at low temperature to enhance the stability of the target protein. The catalytic activity is insensitive to organic solvents (up to 10%); however, it can be strongly stimulated by high concentration of anions such as sulfate.

References :

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